Journal: Haematologica

Article Title: Impact of Sox11 over-expression in Ba/F3 cells

doi: 10.3324/haematol.2018.197467

Figure Lengend Snippet: Phenotypical, proliferative and transcriptional changes in Ba/F3 cells upon 72 h of induced Sox11 expression. (A) Western Blot of SOX11 protein expression in Sox11-ON (doxycycline supplemented medium) and Sox11-OFF (control medium) cells at 72 h, detected with the rabbit polyclonal anti-SOX11 antibody HPA000536, Sigma-Aldrich. B) Bright field microscopy images of cell aggregates following 72 h of continuous Sox11 expression (10x), imaged by Nikon Ti-E microscope. C) Sox11-ON cells incorporates less 3H-Thymidine at 72 h of Sox11 induction following a 4 h pulse, as compared to Sox11-OFF cells, measured in counts per minute, error bars represent the standard deviation (P=0.0086, n=3). D) Mean relative absorption measured by XTT (n=3). Metabolic activity is significantly reduced (P=0.0124) in Sox11-ON cells as compared to Sox11-OFF cells. E) Volcano plot representation of transcript level differences by Affymetrix MTA-1 mouse arrays (Microarray data has been made available through the GEO database with accession number GSE108419). Names are shown for the genes with the largest transcript level fold change (log2FC ≥1.3 or ≤−1.3). Blue and red: genes with significantly altered transcript levels in Sox11-ON cells and a fold change below -2 (blue) (FDR q-value ≤0.05 and log2FC ≥−1) or above 2 (red) (FDR q-value ≤0.05 and log2FC ≥1); gray: genes significantly changed at the transcript level (FDR q-value ≤0.05); black: genes with non-significant transcript level changes. F) Expression levels after Sox11 induction in genes specifically expressed at different stages of B-cell development. Only the pro-B restricted genes Id1 and Tal1 had significantly altered transcript levels in Sox11-ON cells (FDR q-value: 0.006 and 0.016, respectively). None of the other investigated pro-B and pre-B cell associated genes were altered at the transcript level. Genes associated with later B-cell developmental stages are shown for comparison. Transcript levels are presented as a gene-wise standardized expression (Z-score). FC: fold change.

Article Snippet: E) Volcano plot representation of transcript level differences by Affymetrix MTA-1 mouse arrays (Microarray data has been made available through the GEO database with accession number GSE108419).

Techniques: Expressing, Western Blot, Microscopy, Standard Deviation, Activity Assay, Microarray

Journal: Frontiers in Neuroscience

Article Title: Awake Rat Brain Functional Magnetic Resonance Imaging Using Standard Radio Frequency Coils and a 3D Printed Restraint Kit

doi: 10.3389/fnins.2018.00548

Figure Lengend Snippet: A custom-made 3D printed rat restraint kit (A) , a rat restrained in the bed with the developed restraint kit (B) , and a restrained rat in the bed with a Bruker quadrature receiver coil attached (C) . The rat restraint kit (A) consists of a standard Bruker rat bed (A1) , a padded shoulder/neck support (A2) , a sliding sledge including a bite bar (A3) , padded cheek supports (A4) , and a padded nose cone (A5) . A replicate of Bruker bed was used in mock scanner.

Article Snippet: Our method introduces a 3D printed rat restraint kit compatible with a standard Bruker rat MRI bed, and quadrature surface receiver and transmitter coils.

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Awake Rat Brain Functional Magnetic Resonance Imaging Using Standard Radio Frequency Coils and a 3D Printed Restraint Kit

doi: 10.3389/fnins.2018.00548

Figure Lengend Snippet: Different rat restraint and habituation methods from literature.

Article Snippet: Our method introduces a 3D printed rat restraint kit compatible with a standard Bruker rat MRI bed, and quadrature surface receiver and transmitter coils.

Techniques:

Journal: Biosensors

Article Title: Development of a Direct Non-Puncture Device for Measuring Portal Venous Pressure during Liver Transplantation—A Swine Model

doi: 10.3390/bios13121007

Figure Lengend Snippet: ( a ) Illustration of the surgical anatomy during liver transplantation. We used this space (white arrow under the portal vein) to design the corresponding device. ( b – d ) Simulation of the prototype PVPMD using 3D modeling software, with the corresponding 3D–printed components produced by a 3D Printer UP Mini 2. ( b ) The support base of the PVPMD can be inserted into the space beneath the portal vein; ( c ) The plastic portion of the adjustable bag sleeve was mounted on the support base; and ( d ) the adjustable bag sleeve can be inflated and deflated via an outer plastic tube (black arrow).

Article Snippet: We were confined to the space left below the portal vein for designing the corresponding device. b–d show the prototype PVPMD designed using online 3D modeling software (Sketchup 2016) and created with a thermoplastic polyester-polylactide using a 3D printer (UP Mini 2, Tiertime, Williamsburg, VA, USA).

Techniques: Transplantation Assay, Software, Produced